CJRTP

Chinese Journal of Rehabilitation Theory and Practice >

2018 , Vol. 24 >Issue 7: 802 - 806

DOI: https://doi.org/10.3969/j.issn.1006-9771.2018.07.008

Orignal Article

Role of Ski in Inflammatory Factor Release in Activated Astrocytes

  • WANG Ming ,
  • YANG Xin-le ,
  • CHEN Tie-ge ,
  • ZHANG Dong-liang ,
  • GONG Zhao-yang ,
  • XIANG Gao ,
  • LIU Kai-xin ,
  • ZHANG Hai-hong
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  • 1. Department of Orthopedics, the Second Clinical Medical College of Lanzhou University, Lanzhou, Gansu 730030, China;
    2. Lanzhou University Key Laboratory of Orthopedics of Gansu Province, Lanzhou, Gansu 730030, China

Received date: 2018-04-19

  Revised date: 2018-05-17

  Online published: 2018-08-01

Supported by

Supported by Gansu Natural Science Foundation (No. 17JR5RA238) and the Second Hospital of Lanzhou University Funding (No. CY2017-MS06)

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Abstract

Objective To investigate the effect of Ski on the secretion of inflammatory cytokines from activated astrocytes. Methods Astrocytes were obtained from cerebral cortex of a three-day old Sprague-Dawley rat and cultured in vitro. They were divided into blank group, control group and siRNA group. The Ski gene was silenced in siRNA group. The expression of Ski was tested with Western blotting and immunofluorescence 48 hours later. Then the astrocytes were stimulated with lipopolysaccharide for 24 hours. The secretion of tumor necrosis factor α (TNF-α) and interleukin-1β (IL-1β) in activated astrocytes was detected with ELISA. Results The expression of Ski protein reduced in the siRNA group (P<0.001), as well as the secretion of TNF-α and IL-1β (P<0.001). Conclusion Ski may play a role in inflammatory response of astrocyte.

Key words： Ski; RNA interference; lipopolysaccharide; inflammation; astrocytes; rats

Cite this article

WANG Ming , YANG Xin-le , CHEN Tie-ge , ZHANG Dong-liang , GONG Zhao-yang , XIANG Gao , LIU Kai-xin , ZHANG Hai-hong . Role of Ski in Inflammatory Factor Release in Activated Astrocytes[J]. Chinese Journal of Rehabilitation Theory and Practice, 2018 , 24(7) : 802 -806 . DOI: 10.3969/j.issn.1006-9771.2018.07.008

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