CJRTP

Chinese Journal of Rehabilitation Theory and Practice >

2020 , Vol. 26 >Issue 2: 189 - 196

DOI: https://doi.org/10.3969/j.issn.1006-9771.2020.02.009

Effect of Three Handing-Three Points on Pain Function and Expression of CX3CL1/CX3CR1 in Spinal Cord Dorsal Horn of Rats with Sciatic Nerve Injury

  • Yan-jun MO ,
  • Yu-mo ZHANG ,
  • Tian-yuan YU ,
  • Shuai SHAO ,
  • Yi SHEN ,
  • Yu-ting LUO ,
  • Tao-tao LÜ
Expand
  • Beijing University of Chinese Medicine, Beijing 100029, China

Received date: 2019-09-12

  Revised date: 2019-10-27

  Online published: 2020-03-19

Supported by

National Natural Science Foundation of China(81674094)

Fold

Abstract

Objective To investigate the mechanism of Three Handing-Three Points on pain function in sciatic nerve injury rats by observing the changes of chemokine (C-X3-C motif) ligand 1, CX3CL1)/chemokine (C-X3-C motif) receptor 1 (CX3CR1) protein and mRNA expression in spinal dorsal horn.Methods A total of 74 male Sprague-Dawley rats were randomly divided into normal group (n= 12), sham group (n = 24), model group (n = 25), and Three Handing-Three Points group (Tuina group, n = 13). The model group and Tuina group prepared the sciatic nerve injury model. The sham group exposed sciatic nerve only. Tuina group received Tuina on Yinmen (BL37), Chengshan (BL57) and Yanglingquan (GB34) with Tuina manipulation emulator. The photothermal pain threshold was measured seven days after modeling and after 20 days of intervention; cumulative pain score was measured seven days after modeling, and after ten days and 20 days of intervention. The spinal dorsal horn tissues were extracted to detect the protein and mRNA expression of CX3CL1/CX3CR1 with Western blotting and RT-PCR seven days after modeling and after 20 days of intervention. The microglia morphology in spinal dorsal horn was observed with immunofluorescence after 20 days of intervention.Results Seven days after modeling, compared with the normal group, the photothermal pain tolerance threshold increased in the model group and the sham group (P < 0.05); compared with the sham group, the cumulative pain score increased in the model group and Tuina group ( P < 0.05). After ten days of intervention, the cumulative pain score was lower in Tuina group than in the model group ( P < 0.05). After 20 days of intervention, both the photothermal pain tolerance threshold and cumulative pain score were lower in Tuina group than in the model group ( P < 0.05). There was no significant difference in the expression of CX3CL1/CX3CR1 protein and mRNA on the seven days after modeling and after 20 days of intervention ( P > 0.05). The microglia in the model group were partially activated or completely activated, while those in Tuina group were unactivated or partially activated after 20 days of intervention. Conclusion Three Handing-Three Points can improve the pain function of sciatic nerve injured rats, which may associate with regulating microglia through the pathway other than CX3CL1/CX3CR1.

Key words： sciatic nerve injury; pain function; Tuina; chemokine (C-X3-C motif) ligand 1/chemokine (C-X3-C motif) receptor 1; rats

Cite this article

Yan-jun MO , Yu-mo ZHANG , Tian-yuan YU , Shuai SHAO , Yi SHEN , Yu-ting LUO , Tao-tao LÜ . Effect of Three Handing-Three Points on Pain Function and Expression of CX3CL1/CX3CR1 in Spinal Cord Dorsal Horn of Rats with Sciatic Nerve Injury[J]. Chinese Journal of Rehabilitation Theory and Practice, 2020 , 26(2) : 189 -196 . DOI: 10.3969/j.issn.1006-9771.2020.02.009

References

[1] 李鸿浩. 止痛药会损伤听力吗?[J]. 心血管病防治知识, 2013, 78(6):44.
[2] 刘志林, 陈晓容, 彭丽雅. 药代动力学和药效动力学结合模型对急慢性镇痛药物的评估作用[J]. 保健医学研究与实践, 2016, 13(6):88-91.
[3] 周玉颖, 肖适崎, 孙健. 防治麻醉性镇痛药毒副作用的护理体会[J]. 辽宁药物与临床, 2003, 6(3):145.
[4] 李同军, 刘旭东, 于志国, 等. 五段拔伸推拿法治疗腰椎间盘突出症胫腓神经损伤的临床研究[J]. 针灸临床杂志, 2018, 34(9):63-66.
[5] 吴文刚, 杨清滨, 吴振坤, 等. 推拿治疗周围神经损伤1245例疗效观察[J]. 中国中医药信息杂志, 1999, 6(4):66-67.
[6] 马建强, 李凤岩, 吕忠礼. 推拿手法治疗分娩性臂丛神经损伤的临床观察[J]. 中国中医骨伤科杂志, 2018, 26(3):46-49.
[7] 冼思彤, 于天源, 潘璠, 等. 推拿对坐骨神经损伤大鼠背根神经节降钙素基因相关肽表达的影响[J]. 中医学报, 2015, 30(9):1311-1314.
[8] 鲁梦倩, 于天源, 姚斌彬, 等. 推拿对坐骨神经损伤模型大鼠神经超微结构的影响[J]. 南京中医药大学学报, 2015, 31(4):349-352.
[9] 张林峰, 于天源, 潘璠, 等. 推拿对坐骨神经损伤大鼠髓鞘碱性蛋白表达的影响[J]. 中华中医药杂志, 2016, 31(8):3045-3048.
[10] 高玉峰, 姚斌彬, 吴剑聪, 等. 推拿对大鼠坐骨神经损伤后MAP-2和NF-M表达的影响[J]. 中医药导报, 2014, 20(1):11-13.
[11] 李易真, 杨超, 于天源, 等. “三法三穴”推拿手法对坐骨神经损伤大鼠运动功能和脊髓NogoA及其受体表达的影响[J]. 中国康复理论与实践, 2018, 24(12):1403-1407.
[12] 吴帅, 于天源, 杨超, 等. “三法三穴”对坐骨神经损伤模型大鼠冷感觉超敏反应的行为学研究[J]. 中医药导报, 2017, 23(22):8-11, 22.
[13] Charo I F, Ransohoff R M. The many roles of chemokines and chemokine receptors in inflammation[J]. N Engl J Med, 2006, 354(6):610.
[14] Conroy M J, Maher S G, Melo A M, et al. Identifying a novel role for fractalkine (CX3CL1) in memory CD8(+)T cell accumulation in the omentum of obesity-associated cancer patients[J]. Front Immonul, 2018, 9(3):1867.
[15] Sheridan G K, Murphy K J. Neuron-glia crosstalk in health and disease: fractalkine and CX3CR1 take centre stage[J]. Open Biol, 2013, 3(12):130181-130182.
[16] Zhu W, Acosta C, Macneil B, et al. Elevated expression of fractalkine (CX3CL1) and fractalkine receptor (CX3CR1) in the dorsal root ganglia and spinal cord in experimental autoimmune encephalomyelitis: Implications in multiple sclerosis-induced neuropathic pain[J]. BioMed Res Int, 2013, 1(14):874-880.
[17] Verge G M, Milligan E D, Maier S F, et al. Fractalkine (CX3CL1) and fractalkine receptor (CX3CR1) distribution in spinal cord and dorsal root ganglia under basal and neuropathic pain conditions[J]. Eur J Neurosci, 2004, 20(5):119-123.
[18] Sun J L, Xiao C, Lu B, et al. CX3CL1/CX3CR1 regulates nerve injury-induced pain hypersensitivity through the ERK5 signaling pathway[J]. J Neurosci Res, 2013, 91(4):545-553.
[19] Zhou Q Q, Chen S S, Zhang Q Q, et al. Cerebrospinal fluid-contacting nucleus mediates nociception via release of fractalkine[J]. Braz J Med Biol Res, 2017, 50(9):e6275.
[20] Old E A, Nadkarni S, Grist J, et al. Monocytes expressing CX3CR1 orchestrate the development of vincristine-induced pain[J]. J Clin Invest, 2014, 124(5):2023-2036.
[21] Clark A K, Malcangio M. Microglial signalling mechanisms: Cathepsin S and Fractalkine[J]. Exp Neurol, 2012, 234(2):283-292.
[22] Aldskogius H, Kozlova E. Microglia and neuropathic pain[J]. Glia, 2013, 13(4):2792-2799.
[23] Watkins L R, Milligan E D, Maier S F. Glial activation: a driving force for pathological pain[J]. Trends Neurosci, 2001, 24(8):450-455.
[24] Nair S, Sobotka K S, Joshi P, et al. Lipopolysaccharide-induced alteration of mitochondrial morphology induces a metabolic shift in microglia modulating the inflammatory response in vitro and in vivo[J]. Glia, 2019, 12(5):468-472.
[25] Joana M D, Rita G, Liliana C, et al. Region: pecific control of microglia by adenosine A2A receptors: uncoupling anxiety and associated cognitive deficits in female rats[J]. Glia, 2018, 67(3):278-281.
[26] Owolabi S A, Saab C Y. Fractalkine and minocycline alter neuronal activity in the spinal cord dorsal horn[J]. FEBS Lett, 2006, 580(18):4290-4310.
[27] Begum F, Zhu W, Cortes C, et al. Elevation of tumor necrosis factor alpha in dorsal root ganglia and spinal cord is associated with neuroimmune modulation of pain in an animal model of multiple sclerosis[J]. J Neuroimmune Pharm, 2013, 8(3):677-690.
[28] Scholz J, Woolf C J. The neuropathic pain triad: neurons, immune cells and glia[J]. Nat Neurosci, 2007, 10(11):1361-1368.
[29] Abdo H, Calvo-Enrique L, Lopez J M, et al. Specialized cutaneous Schwann cells initiate pain sensation[J]. Science, 2019, 365(6454):695-699.
Options
Outlines

/

〈 〉