不同电针法对APP/PS1小鼠海马高迁移率族蛋白B1和白细胞介素-10表达的影响

doi:10.3969/j.issn.1006-9771.2018.01.004

Abstract

Abstract: Objective To compare the effect of impulse electroacupuncture (impulse-EA) and music electroacupuncture (music-EA) for Tongdu Qishen on spatial learning and memory, and expression of cytokine in the hippocampus of APP/PS1 mice.Methods A total of 32 APP/PS1 double transgenic male mice were randomly divided into model group (n=8), drug group (n=8), impulse-EA group (n=8) and music-EA group (n=8), the same background and age male C57BL/6 mice were observed as normal group (n=8). The impulse-EA group and music-EA group accepted EA at Baihui (GV20) and Yintang (GV29), connected with their own electroacupuncture stimulators, for 20 minuts, then, they were pricked Renzhong (GV26) for a while. The drug group accepted donepezil hydrochloride 0.92 mg/kg intragastrically. The normal group, model group and drug group were grabbed and bounded in the same way. After 15 days of treatment, they were assessed with Morris water maze. The expression of high mobility group box protein-1 (HMGB1) and interleukin-10 (IL-10) in hippocampus were measured with immunohistochemistry and Western blotting.Results The escape latency shortened inEA groups compared with that of the model group since the fourth day of Morris water maze training (P<0.05), and it was the least in the music-EA group; and the ratio of swimming across the target quadrant increased (P<0.05), but there was no significant difference between EA groups (P>0.05). The expression of HMGB1 decreased (P<0.05) and the expression of IL-10 increased (P<0.05) in EA groups compared with that of the model group, and HMGB1 decreased more and IL-10 increased more in the music-EA group than in the impulse-EA group (P<0.05), according to the measurement in immunohistochemistry, not in Western blotting.Conclusion Both impulse-EA and music-EA based on Tongdu Qishen can promote the recovery of the learning and memory in APP/PS1 mice, and music-EA may do more effectively in inhibition of pro-inflammatory factors and promotion of the anti-inflammatory factors.

Key words: Alzheimer's disease, electroacupuncture, learning and memory, high mobility group box protein-1, interleukin-10, mice

CLC Number:

R749.1

ZHAO Jiang-hao, YAO Hai-jiang, WANG Yuan-zheng, LU Meng-han, LI Yu-jie, SONG Meng, YANG Li-juan, LIU Jun-tong, LI Zhi-gang. Effects of Different Types of Electroacupuncture on Expression of High Mobility Group Box Protein-1 and Interleukin-10 in Hippocampus of APP/PS1 Mice[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(1): 17-22.

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Effects of Different Types of Electroacupuncture on Expression of High Mobility Group Box Protein-1 and Interleukin-10 in Hippocampus of APP/PS1 Mice

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