脊髓损伤患者肠道菌群属水平的特征及意义

doi:10.3969/j.issn.1006-9771.2020.00.003

Abstract

Abstract:

Objective To investigate the changes of genus-level gut microbiome in patients with spinal cord injury and its significance in clinical rehabilitation.Methods Fecal samples were collected from 23 patients with spinal cord injury (patients group) and 21 healthy volunteers (control group). Gut microbiome was detected by 16S rDNA high-throughput sequencing. Bioinformatics methods such as species composition analysis and Random Forest were used to analyze the distribution and difference of genus-level gut microbiome between two groups. Results Compared with the control group, the increased important marker genera in the patients group were as follows: UBA1819, Ruminiclostridium 9, Ruminococcaceae NK4A214 group, Ruminococcus 2, Ruminococceae UCG-005, Ruminiclostridium 5, Flavonifractor belonging to Ruminococceae; Aglistes, dgA-11 gut group, Rikenaceae RC9 gut group belonging to Rikenellaceae; [Eubacterium] oxidoreducens group belonging to Lachnospiraceae; Intestinibacter belonging to Peptostreptococcaceae; Escherichia-Shigella belonging to Enterobacteriaceae; Tannerellaceae belonging to Parabacteroides (|U| > 1.962, P< 0.05). The decreased marker genera in the patients group was Fusobacterium of Fusobacteriaceae (|U| = -2.284, P< 0.05).Conclusion There are significant differences of gut microbiome in spinal cord injury patients. The relative abundance of Ruminococcaceae relating to depression, Ruminococcus relating to central nervous system diseases, and enteropathogenic bacteria such as Escherichia-Shigella and Erysipelothrix increase; and the relative abundance of butyrate-producing bacteria and anti-inflammatory bacteria benefitting to the intestine decrease; which may play a role in clinic.

Key words: spinal cord injury, gut microbiome, clinical rehabilitation

CLC Number:

R651.2

WANG Jun-yu,PANG Ri-zhao,WANG Wen-chun,WANG Mei-yu,XIANG Wu,ZHAO Xiao-hua,ZHANG An-ren. Characteristics of Genus-level Gut Microbiome in Patients with Spinal Cord Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(4): 393-399.

Figures/Tables 5

References 29

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序号	性别	年龄(岁)	病程(月)	损伤平面	ASIA	NBD评分	血液白细胞数(10⁹/L) 参考值(3.5~9.5)×10⁹/L	尿液白细胞(/μl) 参考值(0~25)/μl	尿液细菌(/μl) 参考值(0~1000)/μl
1	女	27	72	L₂	D	8	8.68	24.3	16.3
2	男	32	62	C₄	A	11	8.12	5.3	895.9
3	男	43	48	L₁	D	3	7.48	18.8	184.2
4	男	30	49	C₄	B	4	4.49	27.5	5.7
5	男	28	108	T₁₀	A	11	6.15	17.5	3.8
6	男	24	13	C₄	C	11	7.34	21.0	1195.0
7	男	41	7	C₆	B	20	3.98	24.3	1214.0
8	男	37	6	C₄	C	8	5.11	28.9	665.9
9	男	27	63	C₅	A	11	6.4	3.3	18.2
10	男	36	1	T₄	B	15	6.57	30.9	141.1
11	男	32	3	T₁₀	A	21	6.42	22.7	51.4
12	女	50	2	T₈	A	15	5.36	4.4	4.7
13	男	51	2	T₁₂	A	28	8.13	4.0	0.9
14	男	31	48	T₆	A	8	7.44	34.8	982.0
15	女	44	96	T₁₂	A	8	5.95	16.2	226.0
16	男	45	24	T₁₀	A	11	7.67	31.4	1108.0
17	女	41	300	T₈	A	15	5.83	5.8	23.7
18	男	35	99	T₁₀	A	15	4.84	102.4	519.3
19	女	42	110	T₄	A	11	5.28	21.1	341.9
20	男	39	97	T₁₀	A	11	6.17	54.9	668.3
21	男	42	135	T₁₀	A	3	5.31	22.9	371.5
22	男	26	2	C₄	B	20	6.27	17.2	0.9
23	男	22	2	C₅	A	21	7.27	23.1	848.6

组别	n	性别 (男/女,n)	年龄	NBD评分
对照组	21	16/5	37.05±7.871
患者组	23	18/5	35.87±8.275	12.57±6.298
χ²/t值		0	-0.471
P值		1.000	0.638

属	对照组	患者组	U值	P值
Bacteroides	12.049(7.485, 39.991)	27.987(11.524, 37.853)	-0.905	0.366
Megamonas	1.372(0.209, 26.425)	0.595(0.506, 1.100)	-0.834	0.404
Prevotella 9	0.578(0.249, 29.321)	0.949(0.721, 3.662)	-0.552	0.581
[Eubacterium] coprostanoligenes group	2.034 (0.949, 10.812)	5.540(2.271, 11.321)	-1.701	0.088
Ruminococcaceae UCG-002	3.143(1.066, 7.966)	3.699(1.398, 5.895)	-0.012	0.991
Escherichia-Shigella	0.702(0.632, 1.553)	1.594(0.936, 4.997)	-1.962	0.05
Lachnospiraceae NC2004 group	1.556(0.651, 3.501)	1.007(0.677, 2.416)	-0.341	0.733
Lachnospiraceae UCG-008	0.942(0.398, 1.863)	1.758(1.075, 4.136)	-2.385	0.017
Lachnospiraceae NK4A136 group	1.151(0.468, 4.358)	1.758(0.639, 2.460)	-0.141	0.888
[Eubacterium] eligens group	1.044(0.439, 3.653)	0.506(0.221, 0.797)	-1.774	0.076
Alistipes	0.228(0.126, 0.37)	1.113(0.398, 3.295)	-3.537	< 0.001
Parabacteroides	0.449(0.196, 0.92)	1.651(0.784, 3.588)	-3.302	0.001
Phascolarctobacterium	1.082(0.376,1.619)	1.075(0.133, 2.959)	-0.400	0.690
Ruminococcaceae UCG-014	0.070(0.009, 0.234)	0.082(0.057, 0.126)	-1.340	0.180
Megasphaera	0.829(0.455, 1.049)	1.100(0.651, 1.929)	-1.071	0.284
[Ruminococcus] torques group	0.734(0.338,1.049)	1.436(0.835,1.929)	-2.362	0.018
Enterobacter	0.240 (0.126, 1.837)	0.468(0.139, 1.663)	-0.376	0.707
Roseburia	0.892(0.085, 2.093)	0.487(0.209, 1.479)	-0.505	0.613
Lachnospiraceae UCG-004	0.525(0.289, 0.889)	0.569(0.361, 0.791)	-0.423	0.672
Prevotella 2	0.082(0.013, 1.021)	0.089(0.044, 0.183)	-0.165	0.869
Fusobacterium	0.101(0.016, 0.367)	0.025(0.013, 0.057)	-2.284	0.022

差异菌群	Gini指数	对照组	患者组	U值	P值
UBA1819	0.65	0.019(0, 0.032)	0.133(0.038, 0.285)	-4.227	< 0.001
Ruminiclostridium 9	0.58	0.057(0.028, 0.117)	0.240(0.108, 0.342)	-4.011	< 0.001
Alistipes	0.50	0.228(0.126, 0.379)	1.113(0.398, 3.295)	-3.537	< 0.001
RuminococcaceaeNK4A214 group	0.47	0.171(0.079, 0.250)	0.841(0.304, 1.644)	-3.369	< 0.001
[Eubacterium] oxidoreducens group	0.47	0.025(0.003, 0.054)	0.089(0.038, 0.164)	-3.314	< 0.001
Ruminococcus 2	0.47	0.120(0.411, 0.294)	0.499(0.272, 1.758)	-3.819	< 0.001
Ruminococcaceae UCG-005	0.43	0.076(0.047, 0.262)	0.329(0.196, 1.107)	-3.444	< 0.001
dgA-11 gut group	0.29	0.019(0.003, 0.022)	0.038(0.019, 0.069)	-3.257	0.001
Rikenellaceae RC9 gut group	0.26	0.063(0.016, 0.152)	0.133(0.101, 0.196)	-2.281	0.023
Ruminiclostridium 5	0.23	0.069(0.028, 0.152)	0.171(0.126, 0.247)	-2.882	0.004
Fusobacterium	0.22	0.101(0.016, 0.367)	0.025(0.013, 0.057)	-2.284	0.022
Intestinibacter	0.22	0(0, 0.009)	0.025(0.006, 0.051)	-3.362	< 0.001
Flavonifractor	0.22	0.013(0.006, 0.038)	0.057(0.019, 0.152)	-3.211	0.001
Escherichia-Shigella	0.21	0.702(0.632, 1.553)	1.594(0.936, 4.997)	-1.962	0.050
Parabacteroides	0.21	0.449(0.196, 0.920)	1.651(0.784, 3.588)	-3.302	< 0.001

Characteristics of Genus-level Gut Microbiome in Patients with Spinal Cord Injury

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