异常光周期所致昼夜节律紊乱对大鼠腓肠肌时钟基因和葡萄糖摄取相关基因表达节律的影响

doi:10.3969/j.issn.1006-9771.2022.05.010

Abstract

Abstract:

Objective To investigate the impacts of circadian misalignment on glucose uptake of skeletal muscle and metabolism in rats. Methods A total of 36 male Wistar rats were divided into circadian alignment (CA, normal light-dark cycles, n = 18) and circadian misalignment (CM, shifted light-dark cycles, n = 18) groups. ClockLab behavior analysis was performed for 18 days (61 to 78 days after modeling). Intraperitoneal injection glucose tolerance test and physiologic measures were performed 85 days after modeling. They were euthanized 91 to 92 days after modeling, at 8:00, 12:00, 16:00, 20:00, 24:00 and 4:00 next day. Gastrocnemius tissue was collected and measured for Bmal1, Clock, Per2, Tbc1d1, Glut4 and Pgc1α by reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR). Results The circadian cycle increased (t = -6.557, P < 0.001), the amplitude decreased (t = 2.326, P = 0.030) and the area under curve (AUC) of the blood glucose of intraperitoneal glucose tolerance test decreased (t = -2.622, P = 0.016) in CM group. In gastrocnemius, there was difference in the expression of the Bmal1 (F = 6.691, P < 0.001), Clock (F = 4.188, P = 0.007), Per2 (F = 10.893, P < 0.001), Tbc1d1 (F = 3.411, P = 0.018), Glut4 (F = 5.439, P = 0.002) and Pgc1α (F = 15.376, P < 0.001) across different time; meanwhile, there was difference in the expression of Bmal1 (F = 5.020, P = 0.035), Per2 (F = 8.996, P = 0.006), Tbc1d1 (F = 51.111, P < 0.001) and Pgc1α (F = 10.177, P = 0.004) between groups, and the total Tbc1d1 expression decreased in CM group (t = 4.349, P < 0.001). Conclusion CM induced by shifted light-dark cycles may lead to glucose tolerance impairment in rats, which may be related to the decreased expression of Tbc1d1 and the changes of transcription rhythm of Bmal1, Per2 and Pgc1α in gastrocnemius.

Key words: circadian misalignment, skeletal muscle, glucose tolerance, circadian clock, rats

CLC Number:

R587.1

SONG Shaofei,HOU Yuanyuan,WANG Yunlei,ZHANG Tong. Effects of circadian misalignment induced by abnormal photoperiod on expression rhythm of clock genes and glucose uptake related genes in gastrocnemius in rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 552-558.

Figures/Tables 9

References 40

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组别	n	节律周期/h	节律振幅
CA组	12	24.0±0.1	500.6±120.8
CM组	12	27.0±1.6	412.7±50.2
t值		-6.557	2.326
P值		< 0.001	0.030

组别	n	血糖/mmol·L^-1						AUC
组别	n	0 min	15 min	30 min	60 min	90 min	120 min	AUC
CA组	12	4.7±0.8	14.9±3.3	14.5±4.1	10.4±3.9	8.0±3.5	6.5±2.0	1232.3±362.6
CM组	12	5.2±0.7	19.1±5.1	20.1±6.8	15.0±5.6	10.9±3.2	7.6±1.8	1669.6±449.9
t值		-1.790	-2.445	-2.456	-2.318	-2.159	-1.464	-2.622
P值		0.087	0.024	0.022	0.030	0.042	0.157	0.016

组别	n	体质量/g	腹围/cm	体长/cm	Lee指数
CA组	12	458.1±29.5	19.1±1.0	24.8±3.2	26.5±1.1
CM组	12	468.7±28.9	19.1±1.1	24.9±1.0	26.6±0.5
t值		-0.888	-0.061	-0.086	-0.217
P值		0.384	0.952	0.932	0.831

组别	n	4:00	8:00	12:00	16:00	20:00	24:00	24 h总表达
CA组	3	0.52±0.09	0.89±0.14	0.37±0.16	0.07±0.04	0.11±0.02	0.30±0.11	0.38±0.30
CM组	3	0.10±0.01	0.29±0.09	0.14±0.08	0.52±0.27	0.31±0.18	0.33±0.05	0.28±0.18
t值		7.806	6.351	2.158	-2.881	-2.020	-0.330	1.138
P值		0.001	0.003	0.097	0.045	0.114	0.758	0.263

组别	n	4:00	8:00	12:00	16:00	20:00	24:00	24 h总表达
CA组	3	0.65±0.11	0.79±0.12	1.40±0.41	0.80±0.15	0.68±0.17	0.66±0.16	0.83±0.32
CM组	3	0.47±0.18	0.69±0.08	0.71±0.14	0.74±0.02	0.98±0.23	0.83±0.25	0.74±0.21
t值		1.489	1.188	2.723	0.648	-1.801	-1.000	1.013
P值		0.211	0.301	0.053	0.581	0.146	0.374	0.318

Effects of circadian misalignment induced by abnormal photoperiod on expression rhythm of clock genes and glucose uptake related genes in gastrocnemius in rats

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组别	n	4:00	8:00	12:00	16:00	20:00	24:00	24 h总表达
CA组	3	1.26±0.43	0.41±0.04	1.04±0.04	4.19±0.71	5.19±1.37	2.45±0.47	2.42±1.88
CM组	3	1.00±0.63	1.73±0.73	3.14±0.87	1.27±0.31	1.57±0.80	1.83±0.35	1.76±0.89
t值		0.603	-3.120	-4.171	6.485	3.954	1.804	1.364
P值		0.579	0.088	0.053	0.003	0.017	0.146	0.185

组别	n	4:00	8:00	12:00	16:00	20:00	24:00	24 h总表达
CA组	3	1.16±0.15	0.80±0.22	0.73±0.09	1.12±0.42	2.11±0.76	1.92±0.12	1.31±0.63
CM组	3	0.30±0.09	0.76±0.16	0.87±0.23	0.84±0.20	0.42±0.19	0.42±0.18	0.60±0.28
t值		8.391	0.217	-0.990	1.062	3.716	11.847	4.349
P值		0.001	0.839	0.378	0.348	0.054	< 0.001	< 0.001

组别	n	4:00	8:00	12:00	16:00	20:00	24:00	24 h总表达
CA组	3	1.13±0.02	0.60±0.12	0.86±0.12	0.98±0.27	1.22±0.19	2.74±0.37	1.26±0.74
CM组	3	1.01±0.16	0.92±0.14	1.11±0.20	0.86±0.25	0.77±0.06	1.18±0.65	0.97±0.30
t值		1.386	-2.968	-1.778	0.591	3.955	3.607	1.517
P值		0.295	0.041	0.150	0.586	0.017	0.023	0.143

组别	n	4:00	8:00	12:00	16:00	20:00	24:00	24 h总表达
CA组	3	0.82±0.12	1.14±0.26	1.06±0.05	0.92±0.14	1.28±0.25	1.49±0.20	1.12±0.28
CM组	3	1.22±0.19	1.54±0.13	1.62±0.11	1.09±0.17	0.91±0.06	0.73±0.04	1.18±0.35
t值		-3.112	-2.397	-8.270	-1.317	2.550	6.608	-0.635
P值		0.036	0.075	0.001	0.258	0.063	0.018	0.530

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