Abstract: ObjectiveTo investigate whether intracerebral transplantation of heterogenous neural stem cells(NSCs) may cause immunal rejection or not.MethodsNSCs derived from the brain of embryonic 16-day Wistar rat were cultured in vitro. Cerebral ischemic SD rat models were made by middle cerebral artery occlusion and NSCs were transplanted into the ipsilateral or contralateral caudate nucleus 4 days later. Rats were sacrificed at different time points after transplantation, the reaction and proliferation of mononuclear phagocytic system(MPS) and splenic follicles as well as the survival and migration of NSCs were checked.ResultsNumerous NSCs survived and migrated toward the lesion in vivo. Comparing with control group that did not receive NSCs transplantation, there was no significant infiltration of mononuclear phagocytes or proliferation of splenic follicles in NSCs transplanted rats.ConclusionsIntracerebral transplantation of heterogenous neural stem cells did not cause remarkable immunal rejection.

Key words: stem cells, transplantation, cerebral infarction, immunal rejection