Abstract: ObjectiveTo investigate effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (TSG), on learning and memory function and excitability in Alzheimer's disease rat model induced by cholinergic damage.MethodsBasal forebrain of rats were injured by ibotenic acid injection of 10μg each side. Rats were divided into six groups as the operation, model, positive control drug donepezil hydrochloride, TSG low (0.03g/kg), middle (0.06g/kg) and high (0.12g/kg) dose groups. These drugs were intragastrically administrated for 1 month. The learning and memory function were determined with Morris water maze, channel water maze and step through tests, and the excitability was evaluated by open field analysis. Results TSG (low, middle and high dose)significantly decreased the swimming time and error times (P<0.05) in water maze test and TSG high dose improved the ability of passive avoidance response at step through test.Open field test showed that there was no significant difference on excitability between each group ConclusionTSG can improve learning and memory abilities of Alzheimer's disease rat induced by cholinergic damage, TSG(low and middle dose)improve excitability of central nervous system in Alzheimer's disease rat model,but TSG dose maybe inhibite excitability of central nervous system.

Key words: 4'-tetrahydroxystilbene-2-O-β-D-glucoside (TSG), Alzheimer's disease, animal model, learning and memory function, excitability of central nerver