《Chinese Journal of Rehabilitation Theory and Practice》 ›› 2004, Vol. 10 ›› Issue (01): 17-18.

• 专题 • Previous Articles     Next Articles

Expression of specific proteins of neural cell in amniotic epithelial cells in rats

MENG Xiao-ting, CHEN Dong, LIU Jia-mei, et al   

  1. Department of Histology and Embryology, School of Basic Medical Science, Jilin University, Changchun 130021, Jilin, China
  • Received:2003-12-12 Published:2004-01-25 Online:2004-01-25

Abstract: ObjectiveTo detect specific antigens of neural cells in amniotic epithelial cells(AECs) in rats. MethodsAECs were dissociated and purified from the amnion of pregnancy 12—14 d rats. The expression of specific markers of neural stem cells (Nestin, Musashi) and differentiated cells (MAP-2,NSE,GFAP) and ChAT, NT-3 in the AECs were detected by immunocytochemistry. ResultsThe cultured AECs displayed positive immunoreactivity to MAP-2, NSE, GFAP, Nestin and Musashi. In addition, the cells also demonstrated immunoreactivity to ChAT and NT-3. ConclusionAECs are similar with neural cells and it may be useful as a sustained source to improve outcome of neural stem cells transplantation.

Key words: neural cells, amniotic epithelial cells(AECs), immunocytochemistry, rats