Abstract: Objective To study the effcet of necrosis and apoptosis induced by Brucea javanica oil emulsion on bladder cancer BIU-87 cells. MethodsBrucea javanica oil emulsion of different concentration was added to the bladder cancer BIU-87 cell cultured in vitro. The ratio of apoptosis/necrosis cells and mitochondrial membrane potential(MMP) were detected with flow cytometer. The dissipation of cytochrome C was observed with confocal microscopy. ResultsHigh concentration brucea javanica oil emulsion reduced MMP which led cell necrosis, while that of low concentration diffused the cytochrome C from mitochondria to cytoplasm which induced cell apoptosis. ConclusionHigh concentration brucea javanica oil emulsion mainly causes BIU-87 cells to necrosis, low concentration induces cell to apoptosis.

Key words: brucea javanica, bladder neoplasm, necrosis, apoptosis, mitochondria, cytochrome C