Abstract: ObjectiveTo investigate the condition and method of a long time mesenchymal stem cell (MSCs) culture with outer space cell living system carried by a recoverable satellite.MethodsMSCs were obtained from 1 week New Zealand rabbits, and seeded to culture flask and outer space cell living system. Under the condition of absence O₂, CO₂ and medium unable changed, influence of monolayer culture and outer space cell living system upon survival of long time MSCs culture in simulated space various temperature was observed.ResultsUnder the condition of absence O₂, CO₂ and medium unable changed, monolayer cultured MSCs were maintained to survive for 15 days, and group of space MSCs were successfully maintained to survive for 30 days in outer space cell living system. There was a significant difference between two methods. ConclusionOuter space cell living system can maintain MSCs to survive for 30 days under space flight temperature.

Key words: outer space cell living system, mesenchymal stem cell (MSCs), space flight