Abstract: ObjectiveTo explore the role of tumor necrosis factor related apoptosis inducing ligand (TRAIL) and it's combination with chemotherapeutic agents in treatment of cervical cancer.Methods 100μl of cervical cancer cells suspension were added to each well of 96 well plates. Cells were incubated for 24 hours with TRAIL, Adriamycin (ADM), Mitomycin C (MMC) and TRAIL combined with chemotherapeutic agents at different concentration. Rates of apoptosis and death of cells were detected by flow cytometry.ResultsRates of apoptosis of cells were 20.1% by treated with 100 μg/L TRAIL singly, which was significantly different compared with groups without agents (P<0.01). Inhibitory rates were 36.0% by treated with 10.0mg/L MMC, 44.1% by ADM. However inhibitory rates reached 58.4% or 73.7% by 100.0 μg/L TRAIL +10.0 mg/L MMC or ADM. Combined of TRAIL and MMC, TRAIL and ADM resulted in a synergistic cytotoxic effect (P<0.05).ConclusionIn vitro, TRAIL is an agent for anti cervical cancer by inducing the apoptosis of tumor cell; TRAIL can enhance the effect of ADM and MMC.

Key words: cervical cancer, chemotherapy, tumor necrosis factor related apoptosis inducing ligand (TRAIL)