Abstract: ObjectiveTo observe the activities of cultured neurons incubated with Earle's solution, new minimal essential medium (MEM) or former MEM.MethodsActivity changes of cultured neurons were measured by determining the leakage of lactate dehydrogenase (LDH) and metabolic rate of 3-(4,5-dimethylthiazol)2,5 diphenyltetrazolium bromide (MTT).ResultsActivities of neurons incubated with different culture medium for 12 h or 24 h were significantly different (P<0.01).ConclusionDifferent culture medium can influence neuronal activities.

Key words: neurons, Earle's solution, minimal essential medium (MEM), lactate dehydrogenase (LDH), 3-(4, 5-dimethylthiazol)2, 5-diphenyltetrazolium bromide (MTT)