血清预培养促进神经干细胞的增殖

Abstract

Abstract: ObjectiveTo introduce a method of promoting neural stem cells proliferation.MethodsNeural stem cells were cultured with medium DMEM containing 10% fatal bovine serum for 48 h, then changed medium DMEM/F12 containing epidermal growth factor, basic fibroblast growth factor and B27. Immunohistochemistry was used to identify nestin expression.ResultsNeural stem cells got together fast and formed neurospheres within 48 h and expressed nestin. ConclusionPro-culture with serum can promotes neural stem cell proliferation.

Key words: neural stem cell, pre-culture, serum, proliferation

LI Jun-hua;ZHENG Shu-rong;WAN Hong;et al. Pro-culture with serum promoting neural stem cell proliferation [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2005, 11(05): 339-340.

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Pro-culture with serum promoting neural stem cell proliferation

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