Abstract: ObjectiveTo observe the expression of vascular endothlial growth factor(VEGF) and throbospondin-1(TSP-1) in different grade bladder cancer.MethodsSpecimens of 70 cases of bladder transitional cell cancer including Grade Ⅰ 28 cases,Grade Ⅱ 27 cases,Grade Ⅲ 15 cases(according to WHO pathological grade) were stained with immunohistochemistry method.The expressing values of VEGF and TSP-1 were calculated by mean vascular density(MVD).All data was analyzed with SPSS 10.0,and with expression of VEGF and TSP-1 was detected with immunohistochemisrty double staining.Finally,the result of expression was observed by laser scan with focus microscope.ResultsThere was a positive correlation between the cancer grade and the expression of VEGF,and the expression of Grade Ⅰ was significant difference with that of Grade Ⅱ(P<0.01),but there was no difference between Grade Ⅱ and Grade Ⅲ(P>0.1).Expression of TSP-1 decreased with grade increasing but not showing a negative correlation,while,Grade Ⅰ was significant difference with that of Grade Ⅱ and Grade Ⅲ(P<0.01),Grade Ⅱ was not differcnt with Grade Ⅲ(P>0.1).In 28 Grade Ⅰ cases,values of MVD of VEGF and TSP-1 had no correlation(r_s)=0.167,P>0.1).Double immunostaining detecting with laser scan with focus microcope indicate showed that VEGF and TSP-1 appeared in same bladder cancer specimen.ConclusionExpression of VEGF is gradually increased from Grade Ⅰ to Grade Ⅲ,and VEGF is a promoting factor of angiogenesic bladder cancer.Expression of TSP-1 is the strongest in Grade Ⅰ cases,and TSP-1 is an inhibiting angiogenesic factor,which inhibiting function only in early stage.Immunofluorescent staining can only provide evidence of together expression of VEGF and TSP-1,cannot evaluate the degree of expression.

Key words: bladder cancer, vascular endothlial growth factor(VEGF), thrombospondin-1(TSP-1), immunohistochemistry, mean vascular density, immunofluorescent double staining, laser scan focus microscope