Abstract: ObjectiveTo study effect of Herba Erigerontis on metabolites in cerebral ischemia rats with high-resolution magic angle spinning ¹H nuclear magnetic resonance spectroscopy(HR MAS ¹H NMR). Methods18 male Wistar rats, weighing 150～200 g, were randomly divided into 3 groups such as normal (n=6), ischemia (n=6), Herba Erigerontis treatment (n=6). Rat ischemia model was established with middle cerebral artery occlusion (MCAO), reperfusing or dosed with Herba Erigerontis after 3 h ischemia. The metabolites, including N-acetylaspartate (NAA), creatine (Cre), choline (Cho), glutamate (Glu), and aspartate (Asp) etc., of cerebra and cerebella was observed using HR MAS ¹H NMR. ResultsExcluding Cho, Glu, Asp, the concentrations of most metabolites of rat brain during ischemia were significantly lower than that of normal rats, and could be increased after drug treatment. ConclusionHerba Erigerontis shows positive effect on metabolites in cerebral ischemia rats.

Key words: nuclear magnetic resonance spectroscopy, magic angle spinning, pattern recognition, cerebral ischemia, Herba Erigerontis, rats