《Chinese Journal of Rehabilitation Theory and Practice》 ›› 2007, Vol. 13 ›› Issue (03): 252-254.
• 基础研究 • Previous Articles Next Articles
SONG Ge, YUAN Mei, CUI Xue-mei, et al
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Abstract: Objective To investigate the antitumor immunity induced by tumor derived mixed heat shock protein/peptide(mHSPs),interleukin-12(IL-12)and Cyclophosphamide(Cy).MethodsPurified mixed HSP was prepared from tumor by S180 protein extraction and purification,SDS-PAGE,Western blot and animal experiment were applied for mixed HSPs analysis.ResultsThe proliferation of cytotoxic T lymphocyte(CTL)cocultured in the mHSPs+Cy+IL-12 group was significantly remarkable and the content of CD8+ CTLs was significantly more in comparison with the other groups(P<0.01).To the tumor bearing mice,mHSPs+Cy+IL-12 group showed partial therapeutic efficacy,the averaged survival period was over 60 d,and 90% of the mice in this group got long period tumor free survival(>90d),obvious difference(P<0.05)from the other groups.ConclusionTumor derived mixed HSPs can induce powerful antitumor immune efficacy and show favorable therapeutic efficacy.
Key words: sarcoma-180, neoplasm, immunotherapy, mixed heat shock proteins/peptide complexes, cytotoxic T lymphocyte (CTL), vaccine
SONG Ge;YUAN Mei;CUI Xue-mei;et al. Combined Therapy with Mixed Heat Shock Protein/Peptide Vaccine and Interleukin-12 Results in Enhanced Antitumor Effects [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2007, 13(03): 252-254.
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