《Chinese Journal of Rehabilitation Theory and Practice》 ›› 2007, Vol. 13 ›› Issue (05): 422-424.
• 基础研究 • Previous Articles Next Articles
ZHAO Hui,PAN Jing-kun, LUO yun, et al
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Abstract: Objective To construct a cell line with long time secreting endostatin. Methods Human endostatin cDNA containing interleukin-2 (IL-2) secreting peptide was cloned into eukaryotic expression plasmid pSNA2.0 to construct recombinant plasmid pSNA2.0/Endostatin. The plasmid pSNA2.0/Endostatin was transfected into HEK293 cells by cationic liposome. The positive cell clones were selected by G418 and named hED/293. The expression of endostatin protein was analyzed by Western-blot. Results Endostatin could be determined in the supernatant of hED/293 cells. Conclusion The recombinant eukaryotic expression vector is correctly constructed. The human endostatin protein can be expressed and secreted.
Key words: endostatin, eukaryotic expression vector, HEK293, cell line
ZHAO Hui;PAN Jing-kun;LUO yun;et al. Construction of Recombinant Eukaryotic Expression Vector for Human Endostatin and Its Expression in HEK293 Cells [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2007, 13(05): 422-424.
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