Abstract: Objective To investigate the effects of Morroniside on H₂O₂-induced oxidative injury in SH-SY5Y neuroblastoma cells.MethodsSH-SY5Y cells were pre-incubated with Morroniside(1,10,and 100 μmol/L)for 24 h prior to exposure to H₂O₂(500 μmol/L)for 18 h.The content of reactive oxygen species(ROS),nitric oxide(NO),glutathione(GSH)were determined.ResultsPretreatment the cells with Morroniside(10 and 100 μmol/L)lowered H₂O₂-induced ROS release by 37%(P<0.01)and 27%(P<0.05)versus H₂O₂,and NO release in a dose-dependent manner by 31%(P<0.05),53%(P<0.01)versus H₂O₂.Morroniside(1,10,and 100 μmol/L)increased GSH level in cells treated with H₂O₂ in comparison with cells exposed only to H₂O₂,respectively.ConclusionMorroniside shows neuroprotection effect against H₂O₂-induced oxidation injury in SH-SY5Y cell.

Key words: Morroniside, neurocyte, oxidative stress, nitric oxide, glutathione