Abstract: Objective To observe the differentiation of neural stem cells(NSC)into neurons under different astrocytes feeding layers.MethodsThe NSC purified from primary cultured clones and labeled by DAPI in serum-free media were plated in different feeding layers:cyto-plasma astrocytes and fibrous astrocytes respectively with Neural Basal(NB)media.After 10 d,immunohistochemistry with antibody NF-200 was taken to calculate the percents of neurons by 20 fields of vision chosen randomly.The differentiated neurons were stained with AchE and labeled with Fura-3AM.ResultsThe ratios of differentiated neurons in cyto-plasma and fibrous astrocytes were 72% and 43% respectively.In most differentiated neurons the AchE staining was positive and had the activity of Ca2+ stimulated by medicine.ConclusionThe cyto-plasma astrocytes can induced NSC differentiated into neurons,especially into active motor neurons,which can be chosen for a new seeding cells in the nervous tissue engineering.

Key words: astrocyte, cyto-plasma, fibrous, neural stem cell, neurons, differentiation