Abstract: Objective To investigate the influence of rehmannia glutinosa oligosaccharide(RGOs)on apoptosis of human adipose tissue-derived mesenchymal stromal cells(ADMSCs)induced by hydrogen peroxide(H₂O₂).Methods Cultured human ADMSCs were randomly divided into three groups as the normal group(group N,without any treatment),H₂O₂ group(group H,treated with 0.1 mmol/L H₂O₂),and RGOs group(group R,treated with 0.2g/L RGOs plus 0.1 mmol/L H₂O₂).After 1 h,6 h and 24 h,morphological changes of ADMSCs apoptotic were observed,the apoptotic rate was determined by flow cytometry.Results After 1 h,6 h and 24 h,the apoptotic rate of group H was significantly higher than that of the group N and group R(P<0.05),and the apoptotic rate of group H was significantly higher than that of the group R(P<0.05).Conclusion RGOs can attenuate apoptosis of human ADMSCs induced by H₂O₂.

Key words: adipose tissue-derived mesenchymal stromal cells, rehmannia glutinosa oligosaccharide, apoptosis, hydrogen peroxide