CJRTP

《Chinese Journal of Rehabilitation Theory and Practice》 ›› 2008, Vol. 14 ›› Issue (04): 338-340.

• 基础研究 • Previous Articles     Next Articles

Effects of Tetrahydroxy-stilbene Glycoside on Over-expression of α-synuclein Protein in Transgenic COS-7 Cells

LIU Ying, LI Lin,YANG Hui, et al   

  1. The Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China
  • Received:2008-02-14 Published:2008-04-01 Online:2008-04-01

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Abstract: Objective To explore the effects of 2,3,5,4'-tetrahydroxy stilbene-2-β-D-glycoside(TSG)on the over-expression and aggregation of α-synuclein in vitro.Methods TSG in different concentrations was incubated with α-synuclein transgenic COS-7 cells for 24 h.The cell viability was measured by MTT method.The expression of α-synuclein protein was determined by immunocytochemistry and Western blotting method.Results Incubation of TSG at the range of 12.5～200.0 μmol/L with α-synuclein transgenic COS-7 cells for 24 h did not influence cell viability,but a dose-dependently inhibition for the over-expression of α-synuclein protein could be observed in the tests of immunocytochemistry and Western blotting.Conclusion TSG can inhibit the over-expression of α-synuclein protein in COS-7 cells in vitro.

Key words: 4'-tetrahydroxy stilbene-2-β-D-glycoside(TSG), Polygonum multiflorum, α-synuclein, COS-7 cell, in vitro