Abstract: Objective To investigate the effects of morroniside on hydrogen peroxide (H₂O₂)-induced calcium overload and cytotoxicity in SH-SY5Y neuroblastoma cells. Methods SH-SY5Y cells were pre-incubated with morroniside 1, 10, or 100 μmol/L for 24 h prior to exposure to H₂O₂ 300~500 μmol/L for 18 h. The cytosolic free calcium concentration and LDH release were determined. Results The H₂O₂-induced cytosolic free calcium concentration decreased in the cells pre-incubated with morroniside 10 or 100 μmol/L, while the LDH release level decreased in the cells pre-incubated with morroniside 1, 10 or 100 μmol/L, comparison with the cells exposed H₂O₂ along. Conclusion Morroniside effects neuroprotection against H₂O₂-induced calcium overload and cytotoxicity in SH-SY5Y cell.

Key words: Morroniside, neuroblastoma cell, SH-SY5Y, oxidative stress, calcium overload, cytotoxicity