Abstract: Objective To investigate the effect of adipose tissue-derived mesenchymal stem cells (ADMSCs) transplanted by different Methods on cardiac function of rabbits with dilated cardiomyopathy.Methods 50 white ears rabbits were given doxorubicin by intraperitoneal injection to induce dilated cardiomyopathy, and randomly divided into the model group 1, model group 2 and control group. The ADMSCs isolated and cultured in vitro were transplanted into dilated cardiomyopathy rabbits of model group 1 through multi-points injection in myocardium, and were transplanted into rabbits of model group 2 through coronary artery transplant. Those in the control group were treated with IMEM medium of the same volume through multi-points injection in myocardium. Rabbits were fed for 4 week successively and then were killed to obtain heart sample, and the survival and differentiation of transplanted cells were observed through fluorescence microscope. Before transplantation and 4 weeks after transplantation, all rabbits received ultrasonic cardiogram test and haemodynamics test to determine cardiac function.Results After transplant through the two different Methods , ADMSCs could survive and differentiate in myocardial cells. 4 weeks after transplantation, the left ventricular end-diastolic volume (LVEDV) and left ventricular end-systolic volume (LVESV) reduced more significantly in the model group 2 compared with the model group 1 ( P<0.05～0.01), and LVSV, ejection fraction (EF), left ventricular systolic pressure, +dp/dtmax, -dp/dtmax increased more significantly ( P<0.05～0.01).Conclusion Transplanted ADMSCs can survive and differentiate in myocardial cells, and improve the heart function in rabbits with dilated cardiomyopathy. It is more conducive to the effectiveness of ADMSCs to improve the heart function through the method of coronary artery transplant.

Key words: adipose tissue-derived mesenchymal stem cells, method of transplant, dilated cardiomyopathy, cardiac function