Abstract: ObjectiveTo study the protective effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside(TSG) on the PC12 cells injury induced by H₂O₂.MethodsAll cells were divided into 5 groups: the saline control group(control group), Oxidative damage PC12 cells model group(H₂O₂ group) which was induced by H₂O₂, and TSG treated group, which oxidative damage PC12 cells model which was induced by H₂O₂ after given TSG 2 h at TSG 120 μg/L(TSG 120 μg/L+H₂O₂ group), 60 μg/L(TSG 60 μg/L+H₂O₂ group) and 30 μg/L(TSG 30 μg/L+H₂O₂ group) once. The survival rate of the cells was determined by MTT method, the content of lactate dehydrogenase (LDH) was determined by ultraviolet spectrophometry and the content of malonyldialdehyde (MDA) and superoxide dismutase (SOD) activity was measured respectively by thiobarbituric aicd and xanthine oxidese method. Immunohistochemitry method were used to detect the expression of bcl-2.ResultsTSG reduced obviously cells injury induced by H₂O₂. 30～120 μg/L TSG improved the cells survival rate, reduced LDH releasing and MDA content, increased SOD activity, and decreased the expression of bcl-2 in the PC12 cells injury induced by H₂O₂(P<0-05, P<0-01). ConclusionTSG has significantly potective effect on the PC12 cells injury induced by H₂O₂.

Key words: 4'-tetrahydroxystilbene-2-O-β-D-glucoside(TSG), H₂O₂, PC12, cell injury