葛根素抑制脂多糖诱导RAW264.7巨噬细胞表达诱导型一氧化氮合酶

《Chinese Journal of Rehabilitation Theory and Practice》 ›› 2010, Vol. 16 ›› Issue (9): 835-836.

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Puerarin Inhibits Inductible Nitric Oxide Synthase expression in Lipopolysaccharide -induced RAW264.7 Macrophage Cells

YU Jian, HU Wen-zhi, JI Ling, et al

Department of Cardiology, The Second Affiliated Hospital, Nanjing Medical University, Nanjing 210011, Jiangsu, China

Received:2010-04-02 Revised:2010-08-05 Published:2010-09-25 Online:2010-09-25

Abstract

Abstract: ObjectiveTo investigate the effect of Puerarin on expression of inductible nitric oxide synthase (iNOS) in RAW264.7 macrophage cells induced by lipopolysaccharide （LPS）. MethodsThe protein and mRNA levels of lipopolysaccharide (LPS)-induced iNOS was determined in RAW264.7 macrophage cells using Western blotting and RT-PCR respectively. ResultsPuerarin inhibited expression of iNOS concentration dependently (P<0.01). ConclusionPuerarin may reduce iNOS level, result in prevention of inflammation.

Key words: Puerarin, macrophage cells, lipopolysaccharide, inductible nitric oxide synthase

YU Jian;HU Wen-zhi;JI Ling;et al. Puerarin Inhibits Inductible Nitric Oxide Synthase expression in Lipopolysaccharide -induced RAW264.7 Macrophage Cells[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2010, 16(9): 835-836.

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Puerarin Inhibits Inductible Nitric Oxide Synthase expression in Lipopolysaccharide -induced RAW264.7 Macrophage Cells

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