CJRTP

《Chinese Journal of Rehabilitation Theory and Practice》 ›› 2011, Vol. 17 ›› Issue (2): 141-144.

• 论文 • Previous Articles     Next Articles

Loss of Heterozygosity in Oligodendroglioma by Real-time Quantitative Polymerase Chain Reaction-based Microsatellite Analysis

SU Jiang, JIANG Tao, SUN Zhen-rong, et al.   

  1. Beijing Neurosurgical Institute, Capital University of Medical Sciences, Beijing 100050, China
  • Received:2010-12-10 Revised:1900-01-01 Published:2011-02-25 Online:2011-02-25

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Abstract: ObjectiveTo set up the new lab examination method for 1p, 19q and 10q loss of heterozygosity(LOH) in glioma.MethodsThirty-eight cases of oligodendroglioma were enrolled into the study. Real-time quantitative polymerase chain reaction-based microsatellite analysis was performed on tumor tissues in order to study the status of chromosomes 1p, 19q and 10q.ResultsAmong the 38 cases of oligodendroglioma, 25 cases (65.7%) showed 1p LOH, 26 cases (68.4%) showed 19q LOH, while 5 cases (13.2%) showed 10q LOH.ConclusionReal-time quantitative polymerase chain reaction-based microsatellite analysis is a rapid and specific for detecting LOH in glioma tissues.

Key words: oligodendroglioma, loss of heterozygosity, polymerase chain reaction