CJRTP

《Chinese Journal of Rehabilitation Theory and Practice》 ›› 2015, Vol. 21 ›› Issue (10): 1145-1150.

• 基础研究 • Previous Articles     Next Articles

Differentiation of Spinal Neural Stem Cells from Various Gestational Aged Fetal Rats

NAN Wei1a,2, FENG Dong- liang1a,2, ZHOU Kai- sheng1a,2, ZHU Yan- dong1a,2, WANG Jing1a,2, TIAN Ting1b, JIANG Long3, LONG Zai-yun3, WU Ya-min3, ZHANG Hai-hong1a   

  1. 1. a. Department of Orthopedics; b. Department of Neurology, the Second Clinical Medical College of Lanzhou University, Lanzhou, Gansu 730030, China; 2. Key Laboratory of Orthopedics of Gansu Province, Lanzhou, Gansu 730030, China; 3. State Key Laboratory of Trauma, Burns and Combined Injury, the third Department of Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-10-25 Online:2015-10-25

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Abstract: Objective To study the differentiation and proliferation ability of the spinal neural stem cells (NSCs) at different gestational ages in fetal rats. Methods Sprague-Dawley fetal rats were divided into group A (12 days of pregnancy), group B (14 days of pregnancy) and group C (16 days of pregnancy). NSCs were separated with enzyme-assisted microdissection. The diameter and numbers of NSCs balls were measured at different time. The cell growth curve was drawn with CCK8 colorimeter. NSCs were identified with BrdU/Nestin immunohistochemical staining. They were induced with 10% fetal bovine serum for 10 days, and the expression of β-tubulinⅢ and glial fibrillary acidic protein was detected with immunocytochemistry. Results There were cells expressed BrdU, Nestin, β-tubulinⅢ and GFAP in all the group. The most cells (22.74±0.79%) expressed β-tubulinⅢ in the group B, but no significant difference between group B and group C. The cell vitality on the 5th day of third-generation neural stem cells was the most in group B. Conclusion For enzyme-assisted microdissection, it may obtain more neurons to isolate the neural stem cells from 14 days of pregnancy pregnant rats.

Key words: neural stem cells, spinal cord, neurons, astrocyte, protoplasmic astrocyte, rats