两种组织透明技术在免疫荧光染色观察脊髓3D结构中的应用①

Abstract

Abstract: Objective To compare the application of CUBIC and iDISCO clearing methods in observing 3D imaging of spinal cord with immunofluorescent staining. Methods 1 mm thick spinal cord coronal sections were processed with CUBIC and iDISCO, respectively. The neurofilament (NF) protein was detected by immunofluorescent staining and then was observed by a laser confocal microscope. Results Compared with CUBIC, iDISCO had the advantages of shorter time, higher transparency (F=6.64, P<0.01), and deeper penetration (F= 5117.55, P<0.01). Conclusion Immunofluorescent staining combined with iDISCO could completely observe the spinal axons with shorter time and better stain effect.

Key words: spinal cord, transparency, axon regeneration, CUBIC, iDISCO, rats

DUAN Hong- mei1, SHANG Jun- kui2, HAO Peng2, LI Qian- qian1, YANG Fei- xiang2, YANG Zhao- yang1,2, LI Xiao-guang1,2. Application of Two Kinds of Tissue Clearing Method in Observing 3D Imaging of Spinal Cord with Immunofluorescent Staining[J]. 《Chinese Journal of Rehabilitation Theory and Practice》.

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Application of Two Kinds of Tissue Clearing Method in Observing 3D Imaging of Spinal Cord with Immunofluorescent Staining

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