Objective To explore how and where ski expresses under lipopolysaccharide (LPS) in rats' astrocytes. Methods Astrocytes were obtained from cerebral cortex of a newborn (within 3 days) Sprague-Dawley rat and cultured in vitro. Astrocytes were cultured with LPS in concentration of 0 μg/ml, 0.001 μg/ml, 0.01 μg/ml, 0.1 μg/ml, 1 μg/ml, 10 μg/ml and 100 μg/ml for six hours; and cultured with LPS in concentration of 0.1 μg/ml for 0 day, 2 days, 4 days, 6 days and 8 days. The level of ski was determined with Western blotting, and the location of ski was detected with indirect immunofluorescent staining. Results The expression of ski was induced by LPS, especially in the concentration of 0.1 μg/ml. The expression of ski induced with 0.1 μg/ml LPS peaked at 4 days of inducement and then decreased. Ski was mainly observed in nuclear in the normal astrocytes and the astrocytes induced with 0.1 μg/ml LPS for 6 days. However, it was observed in cytoplasm 2 and 4 days of inducement. Conclusion LPS could induce the expression of ski in rats' astrocytes, which may participate in inflammation.
ZHAO Xin
,
GUO Yong-qiang
,
WANG Ming
,
CHEN Tie-ge
,
WU Ya-min
,
ZHANG Hai-hong
. Effect of Lipopolysaccharide on Ski Expression in Rats' Astrocytes[J]. Chinese Journal of Rehabilitation Theory and Practice, 2017
, 23(5)
: 514
-519
.
DOI: 10.3969/j.issn.1006-9771.2017.05.005
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