Objective To compare the effect of impulse electroacupuncture (impulse-EA) and music electroacupuncture (music-EA) for Tongdu Qishen on spatial learning and memory, and expression of cytokine in the hippocampus of APP/PS1 mice.Methods A total of 32 APP/PS1 double transgenic male mice were randomly divided into model group (n=8), drug group (n=8), impulse-EA group (n=8) and music-EA group (n=8), the same background and age male C57BL/6 mice were observed as normal group (n=8). The impulse-EA group and music-EA group accepted EA at Baihui (GV20) and Yintang (GV29), connected with their own electroacupuncture stimulators, for 20 minuts, then, they were pricked Renzhong (GV26) for a while. The drug group accepted donepezil hydrochloride 0.92 mg/kg intragastrically. The normal group, model group and drug group were grabbed and bounded in the same way. After 15 days of treatment, they were assessed with Morris water maze. The expression of high mobility group box protein-1 (HMGB1) and interleukin-10 (IL-10) in hippocampus were measured with immunohistochemistry and Western blotting.Results The escape latency shortened inEA groups compared with that of the model group since the fourth day of Morris water maze training (P<0.05), and it was the least in the music-EA group; and the ratio of swimming across the target quadrant increased (P<0.05), but there was no significant difference between EA groups (P>0.05). The expression of HMGB1 decreased (P<0.05) and the expression of IL-10 increased (P<0.05) in EA groups compared with that of the model group, and HMGB1 decreased more and IL-10 increased more in the music-EA group than in the impulse-EA group (P<0.05), according to the measurement in immunohistochemistry, not in Western blotting.Conclusion Both impulse-EA and music-EA based on Tongdu Qishen can promote the recovery of the learning and memory in APP/PS1 mice, and music-EA may do more effectively in inhibition of pro-inflammatory factors and promotion of the anti-inflammatory factors.
ZHAO Jiang-hao
,
YAO Hai-jiang
,
WANG Yuan-zheng
,
LU Meng-han
,
LI Yu-jie
,
SONG Meng
,
YANG Li-juan
,
LIU Jun-tong
,
LI Zhi-gang
. Effects of Different Types of Electroacupuncture on Expression of High Mobility Group Box Protein-1 and Interleukin-10 in Hippocampus of APP/PS1 Mice[J]. Chinese Journal of Rehabilitation Theory and Practice, 2018
, 24(1)
: 17
-22
.
DOI: 10.3969/j.issn.1006-9771.2018.01.004
[1] 贾建平. 神经病学[M]. 7版. 北京:人民卫生出版社,2013: 217.
[2] Jia J, Wang F, Wei C, et al. The prevalence of dementia in urban and rural areas of China [J]. Alzheimers Dement, 2014, 10(1): 1-9.
[3] Azizi G, Mirshafiey A. The potential role of proinflammatory and antiinflammatory cytokines in Alzheimer disease pathogenesis [J]. Immunopharmacol Immunotoxicol, 2012,34(6): 881-895.
[4] Pimplikar SW. Neuroinflammation in Alzheimer's disease: from pathogenesis to a therapeutic target [J]. J Clin Immunol, 2014, 34(1 Suppl): 64-69.
[5] Akiyama H, Barger S, Barnum S, et al. Inflammation and Alzheimer's disease [J]. Neurobiol Aging, 2000, 21(3): 383-421.
[6] Teeling JL, Perry VH. Systemic infection and inflammation in acute CNS injury and chronic neurodegeneration: underlying mechanisms [J]. Neuroscience, 2009, 158(3): 1062-1073.
[7] Szekely CA, Breitner JC, Fitzpatrick AL, et al. NSAID use and dementia risk in the Cardiovascular Health Study Role of APOE and NSAID type [J]. Neurology, 2008, 70(1): 17-24.
[8] Breitner JC. NSAIDs and Alzheimer's disease: how far to generalise from trials? [J]. Lancet Neurol, 2003, 2(9): 527.
[9] 谢艺婷,柳维林,陈立典,等. 电针对阿尔茨海默病模型动物认知功能和组织病理学影响的研究进展[J]. 中国康复理论与实践, 2017, 23(5): 539-542.
[10] 王颖,李威,张亢亢,等. 不同频率电针对阿尔茨海默病大鼠学习记忆能力和海马突触的影响[J]. 中国康复理论与实践, 2016, 22(6): 635-639.
[11] 张军,柯开富,邱一华,等. 神经炎症在阿尔茨海默病发病机制中的作用[J]. 南通大学学报(医学版), 2012,32(1): 44-47.
[12] Halliday G, Robinson SR, Shepherd C, et al. Alzheimer's disease and inflammation: a review of cellular and therapeutic mechanisms [J]. Clin Exp Pharmacol Physiol, 2000, 27(1-2): 1-8.
[13] Cunningham C. Microglia and neurodegeneration: The role of systemic inflammation [J]. Glia, 2013, 61(1): 71-90.
[14] Solito E, Sastre M. Microglia function in Alzheimer's disease [J]. Front Pharmacol, 2012, 10(3): 14.
[15] Mazarati A, Maroso M, Iori V, et al. High-mobility group box-1 impairs memory in mice through both toll-like receptor 4 and receptor for advanced glycation end products [J]. Exp Neurol, 2011,232(2): 143-148.
[16] 陈轩,王永红. 小胶质细胞与神经系统发育和阿尔茨海默病的关系[J]. 重庆医科大学学报, 2017, 42(6): 755-758.
[17] 唐丽娜,许小明,李艳红. 炎症因子与阿尔茨海默病的相关性研究进展[J]. 中国老年学杂志, 2016, 36(17): 4378-4380.
[18] 张宪亮. 有氧运动及白藜芦醇对Tg APP/PS1小鼠海马Aβ沉积的影响 [D]. 上海:华东师范大学, 2016.
[19] 叶涛,朱路文,唐强,等. 电针预处理对脑缺血再灌注损伤大鼠神经功能和缺血半暗区Tolls样受体4、核转录因子κB蛋白的影响[J]. 中国康复理论与实践, 2017, 23(7): 745-749.
[20] 宋长明,黄佳,林冰冰,等. 电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马CA1区突触超微结构的影响[J]. 中国康复理论与实践, 2017, 23(7): 750-755.
[21] 纪倩,李志刚,唐银杉,等. 不同电针刺激对慢性应激抑郁模型大鼠行为学及海马谷氨酸转运体的影响[J]. 针刺研究, 2013, 38(3): 202-207, 219.
[22] 纪倩,梅旭晖,唐银杉,等. 音乐电针和脉冲电针对慢性应激抑郁大鼠行为学和海马星形胶质细胞的影响[J]. 中华中医药杂志, 2013, 28(3): 648-651.
[23] 周源,李志刚,周阿剑,等. 音乐电针对阿尔茨海默病模型小鼠学习记忆行为能力和海马CA3区Aβ_(1-42)表达的影响[J]. 上海中医药大学学报, 2015, 29(3): 76-80.
[24] 唐银杉,李志刚,陈万顺,等. 音乐电针和脉冲电针对快速老龄化SAMP8小鼠行为学和血清Aβ蛋白的影响[J]. 中医药学报, 2014, 42(1): 87-90.
[25] 邵千枫,李昱颉,曹瑾,等. “通督启神”法两种电针对APP/PS1小鼠额叶皮层小胶质细胞TNF-α表达的影响[J]. 世界科学技术-中医药现代化, 2016, 18(8): 1327-1333.
