Abstract: Objective To determine the effect of transcranial Doppler ultrasound (TCD) combined with focal mild hypothermia on cerebral infarction treated with thrombolytic unokinase (UK).Methods32 rabbits were randomly divided into the TCD group, UK group, TCD+UK group and hypothermia group with 8 animals in each group. Mean velocities of the middle cerebral artery were recorded after unokinase infusion to estimate whether blood vessels reopened. Meantime, brain temperature of lesion site was tested to find out whether declined to hypothermia level.ResultsThe percentage of recanalization was 87.5% in the hypothermia group, 75% in the TCD+UK group, significantly higher than that in the UK group (37.5%)(P<0.05). After 30 minutes of the rabbit refrigerated at 2 ℃, the putamen temperature declined to 32 ℃～34 ℃.ConclusionApply the regional refrigerstor apparatus to decline the brain temperature to mild hypothermia level during therapeutic time window. TCD combined with focal mild hypothermia can improve the thrombolytic effect of unokinase.

Key words: transcranial Doppler ultrasound, focal mild hypothermia, thrombolysis, unokinase