Chinese Journal of Rehabilitation Theory and Practice ›› 2014, Vol. 20 ›› Issue (6): 533-536.

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Effects of Cluster Scalp Acupuncture Combined with Enriched Environment Stimulationon Proliferationand Migration of Endogenous Neural Stem Cells in Neonatal Rats with Hypoxic-ischemic Brain Damage

LIUBo,ZOUWei,TANGQiang,etal.
  

  1. TheSecondAf-filiatedHospitalofHeilongjiangUniversityofChineseMedicine,Harbin150001,Heilongjiang,China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-06-25 Online:2014-06-25

Abstract: ObjectiveToexploretheeffectofclusterscalpacupuncturecombinedwithenrichedenvironmentalstimulationonprolifera-tionandmigrationofendogenousneuralstemcells(NSCs)inneonatalratswithhypoxic-ischemicbraindamage(HIBD).MethodsHIBD modelwasestablishedwithRicemethod.120ratswererandomlydividedintoshamoperationgroup(A),modelgroup(B),clusterscalpacu-puncturegroup(C),enrichedenvironmentalstimulationgroup(D)andclusterscalpacupuncturecombinedwithenrichedenvironmental stimulationgroup(E)with24ratsineachgroup,eachgroupwasdividedinto3days,7daysand14dayssubgroups.Immunohistochemical methodwasusedtoobservethepositivecellsofBromodeoxyuridine(BrdU)andnestin.ResultsComparedwithGroupB,thepositivecells numberofBrdUandnestininsubventricularzone(SVZ)andcerebralcortexincreasedinGroupsCandDatallthetimepoints(P<0.01), andthenumberwasmoreinGroupEthaninGroupsCandD(P<0.05).ConclusionClusterscalpacupuncturecombinedwithenricheden-vironmentalstimulationcouldpromotetheproliferationandmigrationofendogenousNSCsinSVZandcerebralcortexinneonatalratswith HIBD.

Key words: hypoxic-ischemicbraindamage, clusterscalpacupuncture, enrichedenvironmentalstimulation, proliferation, migration, rats